Cell Culture Laboratory
State-of-the-Art Facility for Cellular Innovation
Orbital Shaker Incubator
The ES-20 Orbital Shaker Incubator by Biosan combines orbital shaking with precise temperature control, facilitating uniform mixing and incubation of samples. Its microprocessor-controlled system ensures consistent temperature regulation, while the orbital shaking mechanism promotes even distribution of nutrients and gases within the culture medium. This makes it particularly suitable for applications requiring controlled agitation and incubation, such as growing cell cultures and conducting biochemical reactions.
Key Features
- Temperature Control: Offers a temperature range from 25 °C to 42 °C, with a stability of ±0.5 °C, ensuring optimal conditions for various biological processes.
- Orbital Shaking: Provides adjustable shaking speeds between 50 to 250 rpm, with a 10 mm orbit diameter, allowing for gentle to vigorous mixing as required.
- User-Friendly Interface: Equipped with an LCD display for easy monitoring and setting of parameters such as temperature, speed, and time.
- Compact Design: The benchtop configuration with a transparent plexiglass chamber allows for easy observation of samples while saving laboratory space.
- Versatility: Compatible with various interchangeable platforms, accommodating different types of laboratory glassware and vessels.
Applications
Microbiology: Cultivation of bacteria and yeast cultures under controlled conditions.
Cell Biology: Growth and maintenance of mammalian and plant cell cultures.
Biochemistry: Enzymatic reactions and protein expression studies requiring consistent agitation and temperature.
Pharmaceutical Research: Drug development processes, including testing the effects of compounds on cultured cells.
Food Industry: Fermentation studies and quality control tests involving microbial cultures.
Refrigerated Laboratory Centrifuge
The LMC-4200R Refrigerated Laboratory Centrifuge by BioSan is a benchtop centrifuge designed to maintain precise temperature control during the centrifugation of biological samples. This feature is crucial for preserving the integrity of temperature-sensitive biomaterials, such as enzymes, proteins, and cells, during sample preparation. The LMC-4200R accommodates various sample containers, including microtest plates, gel cards, and tubes ranging from 10 to 50 ml.
Key Features
Temperature Control: Offers a temperature range from –10 °C to +25 °C, with stable maintenance up to 25 °C below ambient temperature.
Centrifugation Parameters: Adjustable speed settings from 100 to 4200 rpm for tubes (up to 3160 × g) and 100 to 2000 rpm for microtitre plates (up to 560 × g), with a speed setting resolution of 100 rpm.
User Interface: Equipped with a user-friendly LCD display that simultaneously shows set and actual values for speed, temperature, and time.
Safety Features: Includes a metal protection chamber, automatic imbalance detection with emergency shutdown, and a lid lock to ensure safe operation.
Operational Flexibility: Features multiple acceleration and deceleration modes (Slow, Normal, Fast) and the option to disable forced braking for delicate samples.
Rotor Compatibility: Supports a wide range of accessory rotors, including options for 96-well plates, gel cards, and various tube sizes.
Applications
Molecular Biology: Preparation of nucleic acids and proteins where temperature control is essential to prevent degradation.
Cell Biology: Centrifugation of cell cultures and subcellular components under controlled temperatures to maintain viability and functionality.
Clinical Diagnostics: Processing of blood samples and other clinical specimens where temperature-sensitive analytes are present.
Pharmaceutical Research: Formulation and stability testing of temperature-sensitive compounds.
Biochemistry: Enzymatic assays and other biochemical applications requiring precise temperature maintenance during centrifugation.
CO2 Incubator
The EuroClone SafeGrow 188 Direct Heat CO₂ Incubator is a high-performance laboratory incubator designed to provide a stable and contamination-free environment for cell and tissue cultures. Utilizing a fan-less, direct heat system, it ensures uniform temperature and CO₂ levels, making it ideal for sensitive applications such as IVF, hybridoma cultures, and stem cell research. The incubator offers a large usable capacity of 140 liters within a total volume of 188.6 liters, accommodating various culture vessels and experimental setups.
Key Features
Direct Heat System: Employs four independently controlled heating elements on all six sides of the chamber, ensuring temperature uniformity with an accuracy of ±0.1 °C and uniformity of ±0.3 °C.
Advanced CO₂ Control: Utilizes a state-of-the-art infrared (IR) sensor to maintain precise CO₂ levels, independent of humidity fluctuations.
High Humidity Maintenance: Achieves consistent humidity levels of approximately 95% using a 2.5-liter stainless steel water tray heated by the bottom element.
Rapid Recovery: Recovers temperature and CO₂ levels within 5 minutes after a 15-second door opening, minimizing disruptions to cultures.
On-Demand Decontamination: Features a validated 12-hour decontamination cycle at 125 °C, ensuring a sterile environment without the need for chemicals.
User-Friendly Design: Equipped with a large, easy-to-read display and programmable keypad for setting parameters and alarms. The seamless stainless steel interior with rounded corners facilitates easy cleaning and reduces contamination risks.
Applications
Cell and Tissue Culture: Provides optimal conditions for the growth and maintenance of various cell lines and tissue samples.
In Vitro Fertilization (IVF): Ensures a stable environment critical for embryo development and viability.
Hybridoma Production: Supports the fusion and culture of hybrid cells for monoclonal antibody production.
Stem Cell Research: Maintains precise conditions necessary for stem cell proliferation and differentiation.
Pharmaceutical Testing: Facilitates drug efficacy and toxicity studies under controlled conditions.
Inverted Microscope
The ZEISS Primovert Inverted Microscope is a compact, user-friendly microscope designed for the efficient examination of living cells. Its inverted configuration allows for the observation of cells in culture vessels like Petri dishes and flasks, making it ideal for cell culture laboratories. The Primovert supports various contrast techniques, including brightfield, phase contrast, and fluorescence, facilitating the analysis of both stained and unstained specimens.
Key Features
Versatile Contrast Techniques: Supports brightfield, phase contrast, and fluorescence imaging, accommodating a wide range of cell types and labeling methods.
Compact and Ergonomic Design: Its small footprint fits easily into laminar flow hoods, and the adjustable viewing tube enhances user comfort.
Integrated Digital Imaging: Select models feature an integrated 5-megapixel HD camera, enabling image capture and documentation without additional equipment.
Digital Connectivity: Interfaces such as HDMI, USB, and Ethernet allow for seamless integration with computers and networks, facilitating image sharing and analysis.
Energy Efficiency: An automatic standby mode activates after periods of inactivity, conserving energy and extending the lifespan of the light source.
Applications
Cell Biology: Monitoring cell cultures for research and quality control purposes.
Microbiology: Observing microorganisms in culture, including bacteria and yeast.
Pharmaceutical Research: Assessing the effects of compounds on cell viability and morphology.
Clinical Diagnostics: Examining patient-derived cell samples for diagnostic evaluations.
Microbiological Safety Cabinet
The Safemate EZ Class II Microbiological Safety Cabinet by BioAir is engineered to provide a secure and contamination-free environment for handling sensitive biological materials. Compliant with the EN 12469 standard, it ensures protection for the operator, the product, and the surrounding environment by maintaining a sterile workspace through controlled airflow and filtration systems.
Key Features
Advanced Airflow System: Incorporates a state-of-the-art AC motor blower that enhances energy efficiency while maintaining optimal airflow conditions.
Ergonomic Design: Features a 5° sloping front aperture and a V-shaped anti-obstruction front grill to maximize user comfort and prevent airflow blockage.
Safety Enhancements: Equipped with an air and aerosol-tight electrical sliding sash and a UV lamp on the back wall for effective sterilization.
Customizable Utilities: Offers optional gas and vacuum taps that can be retrofitted post-installation, providing flexibility for various laboratory needs.
Real-Time Monitoring: Provides real-time reporting of inflow and downflow air velocities, ensuring consistent performance and safety compliance.
Quiet Operation: Designed for silent operation, maintaining noise levels below 49 dB(A), which is conducive to a comfortable working environment.
Applications
Microbiological Research: Handling and culturing of bacterial, viral, and fungal specimens.
Cell Culture: Preparation and maintenance of cell lines under sterile conditions.
Pharmaceutical Development: Formulation and testing of drugs requiring aseptic environments.
Clinical Diagnostics: Processing of patient samples to prevent cross-contamination.
Biotechnology: Manipulation of genetically modified organisms (GMOs) and other sensitive biological materials.